Phosphoribosylamine—glycine ligase
| Phosphoribosylamine—glycine ligase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
phosphoribosylamine-glycine ligase monomer (fragment), Human | |||||||||
| Identifiers | |||||||||
| EC no. | 6.3.4.13 | ||||||||
| CAS no. | 9032-01-3 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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| Phosphoribosylglycinamide synthetase, N domain | |||||||||
|---|---|---|---|---|---|---|---|---|---|
glycinamide ribonucleotide synthetase (gar-syn) from e. coli. | |||||||||
| Identifiers | |||||||||
| Symbol | GARS_N | ||||||||
| Pfam | PF02844 | ||||||||
| InterPro | IPR020562 | ||||||||
| PROSITE | PDOC00164 | ||||||||
| SCOP2 | 1gso / SCOPe / SUPFAM | ||||||||
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| Phosphoribosylglycinamide synthetase, ATP-grasp (A) domain | |||||||||
|---|---|---|---|---|---|---|---|---|---|
glycinamide ribonucleotide synthetase (gar-syn) from e. coli. | |||||||||
| Identifiers | |||||||||
| Symbol | GARS_A | ||||||||
| Pfam | PF01071 | ||||||||
| Pfam clan | CL0179 | ||||||||
| InterPro | IPR020561 | ||||||||
| PROSITE | PDOC00164 | ||||||||
| SCOP2 | 1gso / SCOPe / SUPFAM | ||||||||
| |||||||||
| Phosphoribosylglycinamide synthetase, C domain | |||||||||
|---|---|---|---|---|---|---|---|---|---|
crystal structure of phosphoribosylamine--glycine ligase (tm1250) from thermotoga maritima at 2.30 a resolution | |||||||||
| Identifiers | |||||||||
| Symbol | GARS_C | ||||||||
| Pfam | PF02843 | ||||||||
| InterPro | IPR020560 | ||||||||
| PROSITE | PDOC00164 | ||||||||
| SCOP2 | 1gso / SCOPe / SUPFAM | ||||||||
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Phosphoribosylamine—glycine ligase, also known as glycinamide ribonucleotide synthetase (GARS), (EC 6.3.4.13) is an enzyme that catalyzes the chemical reaction
- ATP + 5-phospho-D-ribosylamine + glycine ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide
which is the second step in purine biosynthesis. The 3 substrates of this enzyme are ATP, 5-phospho-D-ribosylamine, and glycine, whereas its 3 products are ADP, phosphate, and N1-(5-phospho-D-ribosyl)glycinamide.
This enzyme belongs to the family of ligases, specifically those forming generic carbon-nitrogen bonds.
In bacteria, GARS is a monofunctional enzyme (encoded by the purD gene). The purD genes often contain PurD RNA motif in their 5' UTR. In yeast, GARS is part of a bifunctional enzyme (encoded by the ADE5/7 gene) in conjunction with phosphoribosylformylglycinamidine cyclo-ligase (AIRS). In higher eukaryotes, including humans, GARS is part of a trifunctional enzyme in conjunction with AIRS and with phosphoribosylglycinamide formyltransferase (GART), forming GARS-AIRS-GART.