Otto I, Prince of Anhalt-Aschersleben

Otto I
Seal of Otto I
Prince of Anhalt-Aschersleben
Reign1266–1304
PredecessorHenry II
SuccessorOtto II
Died25 June 1304
Spouse
Hedwig of Breslau
(m. 1283)
IssueOtto II, Prince of Anhalt-Aschersleben
Princess Sophie
Princess Elisabeth
HouseAscania
FatherHenry II, Prince of Anhalt-Aschersleben
MotherMatilda of Brunswick-Lüneburg

Otto I, Prince of Anhalt-Aschersleben (died 25 June 1304) was a German prince of the House of Ascania and ruler of the principality of Anhalt-Aschersleben.

He was the eldest son of Henry II, Prince of Anhalt-Aschersleben, by his wife Matilda, daughter of Otto the Child, Duke of Brunswick-Lüneburg. Otto was named after his maternal grandfather.