2000–01 KNVB Cup
| Tournament details | |
|---|---|
| Country | Netherlands |
| Teams | 86 |
| Defending champions | Roda JC |
| Final positions | |
| Champions | Twente |
| Runners-up | PSV |
| Tournament statistics | |
| Top goal scorer(s) | Jan Vennegoor of Hesselink (8) |
The 2000-2001 KNVB Cup (at the time called Amstel Cup) was the 83rd edition of the Dutch national football annual knockout tournament for the KNVB Cup. 86 teams contested, beginning on 8 August 2000 and ending at the final on 24 May 2001.
Roda JC unsuccessfully defended its 2000 title in the Quarter-finals losing to PSV, 0–2. Twente successfully pursued its 2nd #Final KNVB Cup on 24 May 2001 in De Kuip, Rotterdam losing to PSV, 0–0 (penalty score, 3-4). 45,000 attended.
Twente contested the UEFA Cup.