1965 Primera División de Chile

Campeonato Nacional de Fútbol Profesional
Dates3 April 1965 – 27 February 1966
ChampionsUniversidad de Chile (5th title)
RelegatedCoquimbo Unido
1966 Copa LibertadoresUniversidad de Chile
Universidad Católica
Matches played306
Goals scored1,027 (3.36 per match)
Top goalscorerHéctor Scandolli (25)
Biggest home winUnión Española 8–1 Magallanes
(22 January 1966)
Highest attendance74,522 Universidad Católica 0–0 Universidad de Chile
(18 September 1965)
Total attendance3,046,357
Average attendance9,955
1964
1966

The 1965 Campeonato Nacional de Fútbol Profesional, was the 33rd season of top-flight football in Chile. Universidad de Chile won their fifth title, also qualifying for the 1966 Copa Libertadores.