- Achilles' Heels
- Wooden Stake through the heart. In most modern depictions, this is fatal; in the original folklore, it merely stops the vampire from leaving his coffin. In most of the older stories, one had to use a hammer or a grave digger's shovel to drive the stake in, which meant that vampire stakings mainly happened during the day when the vampire was asleep, but recently, it's become oddly easy to do by hand. Remember, the ribs are there to prevent just such an occurrence. In some cases a special specimen of wood is needed for the stake to be effective, commonly Hawthorn, and occasionally it needs to be blessed or enchanted, but not all vampires are this picky about what goes through their chests.
- Decapitation - and as a bonus point, this one isn't exactly exclusive to vampires; it conveniently works on almost any supernatural creature, and humans too. Then again, so does a stake through the heart...
- Fire - another one that can be used to deal with most other supernaturals and also humans, although it varies between interpretations on just how much you need. Really, the only common Achilles's Heel definitely unique to vampires is...
- Direct sunlight. Originally, they actually had to sleep in their coffin during the day, and sunlight wasn't fatal. They were merely dormant during the day, making it "easy" to sneak up on them. Nowadays, they just hole up inside, and sunlight literally has the power to make them spontaneously combustive. Sometimes this is specifically ultraviolet radiation; sunlight is dangerous, but a light-bulb is not. The most common theory as to the reason for this is because the sun provides life-giving energy, which is the polar opposite of the corruption of death and mockery of life that a vampire is. This can vary with age, either becoming less or more effective over time. Because of its lethality, some vampires choose Suicide by Sunlight.
- Dracula in Bram Stoker's novel was almost unaffected by sunlight; it limited his Shapeshifting powers but he could still walk around, was still super-humanly strong, and definitely wasn't burned. The same goes for other vampires before Stoker's, such as Carmilla and Varney the Vampire.
- An interesting inversion are Arabian vampires. They're active during the day and sleep at night, since people were naturally more afraid of the daytime in the desert. Indeed, given the extreme climate of such places, it was much easier for nomadic clans to travel at night.
- The idea that sunlight isn't fatal has undergone somewhat of a resurgence. The vampires in L.J Smith's Night World series can survive exposure to sunlight, but it inhibits their powers. The vampires in Moonlight can survive exposure to sunlight for a limited amount of time. In some folklore, vampires were actually at their strongest at high noon, when their shadow was at its smallest. They were weakest at dusk, when their shadow was at its longest.
- If they exist in the story, magical weapons or other supernatural creatures might also have special abilities to kill vampires.
- The Vampire Hunter. Someone with a special destiny, equipment, powers, or training for taking on vampires. In some legends, vampires can mate with humans to produce dhampirs, beings with an instinctual hatred for and innate ability to destroy vampires.
- Attempting to cross flowing water (e.g., rivers and oceans). Frequently interpreted to mean vampires can't cross flowing water. The effects of flowing water vary greatly depending on the story. Dracula, for example, could cross running water at the slack or flood of the tide.
- Crosses, but not necessarily other religious symbols. Originally, it had to be a full blown crucifix (that is, a cross with a figure of Jesus on it). In modern renditions, this is usually subject to the power of belief of the wielder, the vampire, both, or neither. For instance, if a character is a sincere Jew, then they could use the Star of David to ward off a vampire. Then you can have a vampire who carries his own crucifix, as he is a believer too, like Henry Fitzroy in Blood Ties. He also prays and goes to confession (he figures that he is subject to the same sins as humans, and needs to do penance for them). Fortunately, he is a Friendly Neighborhood Vampire. In other cases, the religion the symbol represents has to have been around during the vampire's lifetime to have any effect. If a vampire predates all modern religions, don't go reaching for your crucifix.
- Holy water (drinking or total immersion in holy water often IS lethal)
- Communion Host (in Bram Stoker's novel, it was used to seal a crypt and prevent a vampire from entering its coffin at sunrise.)
- White roses / roses in general (might have connection with beliefs that roses will not grow over grave)
- Garlic or Onions, although this was more to ward off vampires, not harm them.
- Mustard seed.
- Silver or otherwise magically augmented weapons and ammunition because silver was thought to be pure and represented the untainted light of a deity. Even more useful on werewolves though.
- Thorns (especially hawthorn) in Middle- and Eastern European folklore
- Wolf's Bane (Aconite), a plant featured prominently in the 1930s Dracula film, but also Foxglove (Digitalis) and Holly Bushes.
- Also, folklore tells us vampires get disoriented (or even driven mad) At the Crossroads, and cannot tell one direction for another. Urban vampires seem to have developed a strong resistance to this weakness, especially those that frequent downtown districts (probably by building up an immunity from all the intersections).
- They cannot enter a home unless invited in by someone. This can range from killing them to simply that they physically can't enter. However, it is still a large disadvantage.
- In some folk lore, vampires are all stricken with a debilitating obsession with numbers, if you throw a quantity of small objects on the ground in front of them (seeds,grain,beads etc.) they will not be able to resist the urge to pick it up and count it, this affords the victim time to either run away or kill the vampire. ("Three! Three mustard seeds! Muha-ha-ha!"). Putting said objects into a vampire's coffin keeps them busy counting as well. Sometimes, the urge is powerful enough that you can force the vampire to expose itself to dawn. Sometimes it is not an "urge" but they are somehow forced to count those objects.
- Mandatory tell-tale.
- No reflection (often because the vampire has no soul, but see below). This sometimes extends to shadows. But it depends on the vampire apparently. In one medium there are several types of vamps who have various weaknesses. In more recent examples this has been 'modernised' in terms of the vampire not being able to be picked up by audio or video recording or transmitting equipment.
- No heartbeat/breath. Sometimes the no breath thing means they can't do mouth-to-mouth resuscitation, but if they can talk, they must be able to take air into their lungs and expel it intentionally. Little logic problem there, but we're talking about vampires so logic should take the day off. However Vampires do not need to breathe, it seems to be a reflex they have left over from being alive hence they can never drown, suffocate, or be poisoned.
- No brain activity, (making them easily recognized by telepaths).
- Physical features, such as being exceedingly pale, having unusual eyes (Glowing Eyes of Doom/Hellish Pupils/Animal Eyes), and, of course, Fangs Are Evil. In folklore, there were numerous physical telltales - eyebrows that met over the nose, fingers all the same length, hair in the center of the palms or backward-facing palms - that are mostly overlooked in modern versions. The original novel-version Dracula has practically all of them. If they can hide some or all of them, dropping the disguise constitutes using Game Face. Sometimes vampires will become more and more human-like in appearance as they consume more blood/live longer. Sometimes... not.
- Body temperature: Vampires, being dead, are almost always at room temperature or colder.
- Immortality
- Technically, they are dead. Pretty spry for a dead guy, though.
- Vampires don't age as we mortals do. Sometimes, this is genuine eternal youth. Sometimes long periods of time undead can result in a pretty inhuman-looking character. Sometimes, they age like us, just at a much slower rate.
- Related, they usually suffer from Creative Sterility in both the biological and artistic sense. They can not beget any children unless it's a male vampire and a live woman, in which case a Dhampyr is the result. They may however be capable of turning a child into a vampire, which results in an ageless Undead Child. If it's a "living" vampire species this is usually waived.
- Rarely, the vampire is immortal but must restore his/her youth by drinking blood. In abstinence, they "age", and immediately begin to grow young after they've fed. This originated with Dracula and with persistent stories about one Elizabeth Bathory's bathing habits.
A work will usually address these baseline rules even if they're not enforced. Sometimes an unused rule will be explained away as a Fake Weakness propagated by the vampires themselves.
Some folklore claim the only way to permanently kill a vampire is to hammer a stake through its heart, shove garlic in its mouth, cut off its head, tear off its ears, dismember it, burn the pieces in a fire, and then scatter the ashes across holy ground. This will also permanently kill most anything, including pale spooky goths who happen not to be vampires. A few old folklore suggest that even this only works until a full moon shines on the ash. All this on the theory that vampires were corpses animated by evil spirits. Doing all these things rendered the corpse unusable by the spirit. By contrast, the easiest supposed way to stop a vampire is finding his coffin and turning him face down to make him "bite the dust, not people".
The purpose of vampires in the story varies quite widely. They serve as the Big Bad or as a metaphor for something (communicable diseases like plagues or STDs; alcoholism, drug addiction, denial of aging). There is some danger of the vampire character being too on-the-nose for the metaphor.
The "baseline rules" above are strongly influenced by Hollywood tradition, and not "real" vampire folklore, or even classic vampire fiction. For instance, as (properly) shown in the 1992 Dracula with Gary Oldman and Winona Ryder, and in 2003's League of Extraordinary Gentlemen, Dracula and other "folkloric" vampires were at the most inconvenienced by sunlight, not killed instantly.
In Stoker's novel and earlier vampire lore, sunlight did not cause vampires to go up like flash paper. Several times in the novel, Dracula appears in broad daylight with no ill effects. He is simply incapable of using at least some of his vampiric powers during the daylight (he cannot change form except at dawn, noon and dusk, but still seems to be able to charm wolves to some degree). Sunlight causing a vampire to suffer pain and damage, glitter, smolder, or go up like a one man pyrotechnic band was pretty much wholly created by Hollywood, and specifically, by F.W. Murnau in Nosferatu, the first film to use this idea and probably its inventor (which incidentally was made in 1922 in Germany, when a typical Hollywood film consisted of Buster Keaton being chased by a bunch of coppers).
Note that having a heroic vampire no longer counts as "different". Vampire Refugees are also a frequently used trope.
Differences may be reinforced by spelling it "Vampir" or "Vampyre", or using a clever synonym like "nosferatu" "sanguinarian" or "strigoi". If the differences are emphasized by overt mocking of other authors and unused vampire tropes it becomes Your Vampires Suck.